Sanjay Adhikari, PhD

Biochemist

Dr. Adhikari is an Assistant Professor at Georgetown Lombardi Comprehensive Cancer Center, where he has been a faculty member since 2008. He has a PhD in Biochemistry and Biophysics from Calcutta University, India. He completed his postdoctoral training in base excision repair at Lombardi. As an independent principal investigator he is interested in the repair of etoposide mediated DNA-protein crosslinks. DNA topoisomerases II (TopII) maintain normal DNA topology in cells. The TopII family is a popular target for many anticancer drugs known as topo-poisons, which generate an irreversible TopII-DNA crosslink. TopII produces 5′ DNA-protein crosslinks in both strands simultaneously. However, the repair of TopII-DNA complexes is poorly understood. Recently, a human 5′-tyrosine phosphodiesterase (hTDP2) has been identified for the excision of TopII-DNA adducts. Knockdown/knockout of TDP2 in A549 and DT40 cells increased sensitivity to the TopII targeting agent etoposide. Dr. Adhikari is interested elucidating the regulatory mechanism and interacting protein partner of TDP2 in cancer vs. normal cells. Also, he is interested in learning the effect of polymorphisms in the TDP2 gene and their effect on cancer chemotherapy resistance.